Wow. Very nice crop. And I love the depth of focus here. It's not from front to back but side to side (if that makes sense) and it blurs the background quite nicely without making me think "oh gosh another small DOF just because it's a macro shot". Personally I wish more of the spider had been in focus but I think that's just personal preference and quite minor really.
Right then, joke's on me as I now have to plough through 564 images and bump them all (yes, all of them) up. There'll be a third run through for fine tuning. It's day two now and I'm running late, sorry.
I'm fine with this spider not being centered (being centered is overdone sometimes),
but it does have to be in focus. And this spider isn't, or not enough.
The background doesn't need to be focused, and in fact adds DOF if
it was blurred, but the subject does have to be in focus.