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01/08/2013 07:51:35 PM · #1 |
Because how cool is it to be able to see these? I think he's adorable :-) |
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01/08/2013 07:58:46 PM · #2 |
He is rather cute - sort of looks like a vacuum cleaner bag with legs. Microscope photography would be a fascinating thing to do.
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01/08/2013 08:03:26 PM · #3 |
Originally posted by jomari: He is rather cute - sort of looks like a vacuum cleaner bag with legs. Microscope photography would be a fascinating thing to do. |
It is fun to do, but due to the crazy narrow DOF of the objectives, focus stacking using an automated stage is almost a given to get decent images....The only exception is very flat objects. |
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01/08/2013 08:08:02 PM · #4 |
Originally posted by bassbone: Originally posted by jomari: He is rather cute - sort of looks like a vacuum cleaner bag with legs. Microscope photography would be a fascinating thing to do. |
It is fun to do, but due to the crazy narrow DOF of the objectives, focus stacking using an automated stage is almost a given to get decent images....The only exception is very flat objects. |
There may be another way to get awesome shots. |
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01/08/2013 08:08:46 PM · #5 |
Originally posted by Art Roflmao: Originally posted by bassbone: Originally posted by jomari: He is rather cute - sort of looks like a vacuum cleaner bag with legs. Microscope photography would be a fascinating thing to do. |
It is fun to do, but due to the crazy narrow DOF of the objectives, focus stacking using an automated stage is almost a given to get decent images....The only exception is very flat objects. |
There may be another way to get awesome shots. |
Shhh! I told you that was our secret.... |
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01/08/2013 08:19:16 PM · #6 |
Originally posted by bassbone: Originally posted by jomari: He is rather cute - sort of looks like a vacuum cleaner bag with legs. Microscope photography would be a fascinating thing to do. |
It is fun to do, but due to the crazy narrow DOF of the objectives, focus stacking using an automated stage is almost a given to get decent images....The only exception is very flat objects. |
It is worth noting here that a P&S is often superior to a DSLR in this respect. |
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01/08/2013 09:03:23 PM · #7 |
Originally posted by Cory: Originally posted by bassbone: Originally posted by jomari: He is rather cute - sort of looks like a vacuum cleaner bag with legs. Microscope photography would be a fascinating thing to do. |
It is fun to do, but due to the crazy narrow DOF of the objectives, focus stacking using an automated stage is almost a given to get decent images....The only exception is very flat objects. |
It is worth noting here that a P&S is often superior to a DSLR in this respect. |
Most of the scopes we use have specialized CCD cameras that are built specifically for the scopes themselves...and as Cory points out below...the sensor size of those cameras is very small to help increase DOF.
Message edited by author 2013-01-08 21:13:35. |
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01/08/2013 09:07:27 PM · #8 |
Originally posted by bassbone: Originally posted by Cory: Originally posted by bassbone: Originally posted by jomari: He is rather cute - sort of looks like a vacuum cleaner bag with legs. Microscope photography would be a fascinating thing to do. |
It is fun to do, but due to the crazy narrow DOF of the objectives, focus stacking using an automated stage is almost a given to get decent images....The only exception is very flat objects. |
It is worth noting here that a P&S is often superior to a DSLR in this respect. |
Most of the scopes we use have specialized CCD cameras that are built specifically for the scopes themselves... |
The smaller the sensor, the greater the DOF (at least in theory)... |
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01/08/2013 09:11:06 PM · #9 |
Originally posted by Cory: =
The smaller the sensor, the greater the DOF (at least in theory)... |
Good point...I always seem to forget the influence of sensor size.... |
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01/08/2013 09:56:13 PM · #10 |
Originally posted by Cory: The smaller the sensor, the greater the DOF (at least in theory)... |
This doesn't really hold true when using a microscope. DoF for microscopes is a different affair. There are two components to microscope DoF:
Diffraction-limited DoF = (Lambda*n)/NA^2
Geometrical Optical DoF = (n/(M*NA))*e
In practice, diffraction-limited DoF dominates. It is dependent only on lambda (wavelength of light), n (refractive index of medium between objective and subject, air = 1.0) and NA, the numerical aperture of the objective. If you're unfamiliar with numerical aperture, it's basically the relationship between the size of the front element and the distance to the subject. Bigger element and/or shorter working distance = higher NA.
ETA: The Tardigrade image loks like a colorized SEM image. A SEM, for those unfamiliar, is a Scanning Electron Microscope. It has very great depth of field and resolution far beyond optical microscopes.
Message edited by author 2013-01-08 21:57:51. |
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01/08/2013 10:19:22 PM · #11 |
Originally posted by kirbic:
ETA: The Tardigrade image loks like a colorized SEM image. A SEM, for those unfamiliar, is a Scanning Electron Microscope. It has very great depth of field and resolution far beyond optical microscopes. |
All that power and it doesn't shoot in color...??????? |
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01/08/2013 10:24:10 PM · #12 |
Amongst all the very cool stuff that Dan Winters has done, he did some honey bee photos [url=//danwintersphoto.com/#/H O N E Y B E E S/Overview_HB/1/caption]//danwintersphoto.com/#/H O N E Y B E E S/Overview_HB/1/caption[/url] with an SEM. They don't reproduce all that well on the website- they're much better in his book.
ETA: Also, tardigrades are awesome and provide some interesting reading. Here's a brief bit inDiscover Magazine
ETA2: I don't know why that first one won't parse... it's the right url.
Message edited by author 2013-01-08 22:30:47. |
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01/09/2013 12:44:36 AM · #13 |
Originally posted by spiritualspatula: ETA2: I don't know why that first one won't parse... it's the right url. |
It has spaces in it. Replace them with %20
//danwintersphoto.com/#/H%20O%20N%20E%20Y%20B%20E%20E%20S/Overview_HB/1/caption
Message edited by author 2013-01-09 00:44:41. |
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