Andrea, I think you are on the right track with trying to separate the bud from the background and bring out the colors. the partial desaturation of the greens works well, IMO.
The sharpening seems harsh, though, so you may want to reduce the amount. If you're using the unsharp mask tool, try applying it with radius = 0.4px, amount = 50% and repeat a couple times.
Increasing the contrast is also a vey good idea on this shot, however watch your highlights, they are getting overexposed. BTW, I like your composition on this shot. When you compose a shot like this, even tho the background is out of focus, watch it carefully, sometimes a small change in camera position can significantly improve a background.
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